Essential role for STAT3/FOXM1/ATG7 signaling-dependent autophagy in resistance to Icotinib.

ABSTRACT:

Background

The contribution of autophagy to cancer therapy resistance remains complex, mainly owing to the discrepancy of autophagy mechanisms in different therapy. However, the potential mechanisms of autophagy-mediated resistance to icotinib have yet to be elucidated.

Methods

The effect of autophagy in icotinib resistance was examined using a series of in vitro and in vivo assays. The results above were further verified in biopsy specimens of lung cancer patients before and after icotinib or gefitinib treatment.

Results

Icotinib increased ATG3, ATG5, and ATG7 expression, but without affecting Beclin-1, VPS34 and ATBG14 levels in icotinib-resistant lung cancer cells. Autophagy blockade by 3-MA or silencing Beclin-1 had no effects on resistance to icotinib. CQ effecti

SUBMITTER: Lyu X 

PROVIDER: S-EPMC9188165 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature