TGF-β orchestrates the phenotype and function of monocytic myeloid-derived suppressor cells in colorectal cancer.

ABSTRACT:

Background

Monocytic myeloid-derived suppressor cells (M-MDSCs) are significantly expanded in the blood of colorectal cancer (CRC) patients. However, their presence and underlying mechanisms in the tumour microenvironment of CRC have not been examined in detail.

Methods

Tumour tissues and peripheral blood from CRC patients were analysed for the presence of M-MDSCs. The mechanisms of suppression were analysed by blocking pathways by which MDSCs abrogate T cell proliferation. Co-culture of CRC cells with monocytes were performed with and without cytokine blocking antibodies to determine the mechanism by which CRC cells polarise monocytes. Multi-spectral IHC was used to demonstrate the intra-tumoral location of M-MDSCs.

Results

Tumour tissues and blood of CRC patients co

SUBMITTER: Gneo L 

PROVIDER: S-EPMC9188538 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature