Identification of multiple isoforms of glucocorticoid receptor in nasal polyps of patients with chronic rhinosinusitis.

ABSTRACT:

Background

The conventional belief that glucocorticosteroid (GC) acts through a single brand glucocorticoid receptor (GR)α protein has changed dramatically with the discovery of multiple GR isoforms. We aimed to evaluate whether multiple GR protein isoforms are expressed in chronic rhinosinusitis with nasal polyps (CRSwNP) and whether GR protein isoform expression profiles differ between different endotypes of CRSwNP.

Methods

Thirty-eight patients with CRSwNP and ten healthy volunteers were included. The protein expression of multiple GR isoforms in nasal polyps (NPs) tissue and control mucosae was examined by western blot analysis with different GR antibodies.

Results

Five bands, including three bands for known proteins (GRα-A/B, GRα-C, and GRα-D) and two bands for u

SUBMITTER: Shao S 

PROVIDER: S-EPMC9188707 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature