DiMeLo-seq: a long-read, single-molecule method for mapping protein-DNA interactions genome wide.

ABSTRACT: Studies of genome regulation routinely use high-throughput DNA sequencing approaches to determine where specific proteins interact with DNA, and they rely on DNA amplification and short-read sequencing, limiting their quantitative application in complex genomic regions. To address these limitations, we developed directed methylation with long-read sequencing (DiMeLo-seq), which uses antibody-tethered enzymes to methylate DNA near a target protein's binding sites in situ. These exogenous methylation marks are then detected simultaneously with endogenous CpG methylation on unamplified DNA using long-read, single-molecule sequencing technologies. We optimized and benchmarked DiMeLo-seq by mapping chromatin-binding proteins and histone modifications across the human genome. Furthermore, we ide

SUBMITTER: Altemose N 

PROVIDER: S-EPMC9189060 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature