Barrier Perturbation in Porcine Peyer's Patches by Tumor Necrosis Factor is Associated With a Dysregulation of Claudins.

ABSTRACT: The proinflammatory cytokine tumor necrosis factor (TNF) has been described as one of the main mediators of intestinal inflammatory diseases, affecting the composition of tight junction (TJ) proteins and leading to a disruption of the epithelial barrier. An intact intestinal barrier is mandatory, because the follicle-associated epithelium of Peyer's patches represents the first defense line of the intestinal immune system and ensures a controlled uptake of antigens from the gut lumen. In the current study, we have analyzed the detailed effects of TNF on the follicle-associated epithelium of porcine Peyer's patches by applying the Ussing chamber technique. Epithelial tissue specimens of Peyer's patches and the surrounding villus epithelium were mounted into conventional Ussing chambers and

SUBMITTER: Droessler L 

PROVIDER: S-EPMC9189282 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature