Evaluation of Untargeted Metabolomic Strategy for the Discovery of Biomarker of Breast Cancer.

ABSTRACT: Discovery of disease biomarker based on untargeted metabolomics is informative for pathological mechanism studies and facilitates disease early diagnosis. Numerous of metabolomic strategies emerge due to different sample properties or experimental purposes, thus, methodological evaluation before sample analysis is essential and necessary. In this study, sample preparation, data processing procedure and metabolite identification strategy were assessed aiming at the discovery of biomarker of breast cancer. First, metabolite extraction by different solvents, as well as the necessity of vacuum-dried and re-dissolution, was investigated. The extraction efficiency was assessed based on the number of eligible components (components with MS/MS data acquired), which was more reasonable for metaboli

SUBMITTER: Ruan X 

PROVIDER: S-EPMC9189413 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature