Single-cell analysis reveals the Comma-1D cell line as a unique model for mammary gland development and breast cancer.

ABSTRACT: The mammary gland epithelial tree contains two distinct cell populations, luminal and basal. The investigation of how this heterogeneity is developed and how it influences tumorigenesis has been hampered by the need to perform studies on these populations using animal models. Comma-1D is an immortalized mouse mammary epithelial cell line that has unique morphogenetic properties. By performing single-cell RNA-seq studies, we found that Comma-1D cultures consist of two main populations with luminal and basal features, and a smaller population with mixed lineage and bipotent characteristics. We demonstrated that multiple transcription factors associated with the differentiation of the mammary epithelium in vivo also modulate this process in Comma-1D cultures. Additionally, we found that only

SUBMITTER: Werner RL 

PROVIDER: S-EPMC9189430 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature