Tracking and characterization of partial and full epithelial-mesenchymal transition cells in a mouse model of metastatic breast cancer.
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ABSTRACT:
SUBMITTER: Luond F
PROVIDER: S-EPMC9189628 | biostudies-literature | 2022 Jun
REPOSITORIES: biostudies-literature
Publications
Tracking and characterization of partial and full epithelial-mesenchymal transition cells in a mouse model of metastatic breast cancer.
Lüönd Fabiana F Santacroce Natascha N Beisel Christian C Guérard Laurent L Bürglin Thomas R TR Christofori Gerhard G Sugiyama Nami N
STAR protocols 20220610 2
The various stages of epithelial-mesenchymal transition (EMT) generate phenotypically heterogeneous populations of cells. Here, we detail a dual recombinase lineage tracing system using a transgenic mouse model of metastatic breast cancer to trace and characterize breast cancer cells at different EMT stages. We describe analytical steps to label cancer cells at an early partial or a late full EMT state, followed by tracking their behavior in tumor slice cultures. We then characterize their trans ...[more]
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