Localization of T cell clonotypes using the Visium spatial transcriptomics platform.

ABSTRACT: We present a protocol to localize T cell receptor clones using the Visium spatial transcriptomics platform. This approach permits simultaneous localization of both gene expression and T cell clonotypes in situ within tissue sections. T cell receptor sequences identified by this protocol are readily recapitulated by single-cell sequencing. This technique enables detailed studies of the spatial organization of the human T cell repertoire, such as the localization of infiltrating T cell clones within the tumor microenvironment. For complete details on the use and execution of this protocol, please refer to Sudmeier et al. (2022).

SUBMITTER: Hudson WH 

PROVIDER: S-EPMC9189629 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature