Low-Energy (5-20 eV) Electron-Induced Single and Double Strand Breaks in Well-Defined DNA Sequences.

ABSTRACT: Ionizing radiation is used in cancer radiation therapy to effectively damage the DNA of tumors. The main damage is due to generation of highly reactive secondary species such as low-energy electrons (LEEs). The accurate quantification of DNA radiation damage of well-defined DNA target sequences in terms of absolute cross sections for LEE-induced DNA strand breaks is possible by the DNA origami technique; however, to date, it is possible only for DNA single strands. In the present work DNA double strand breaks in the DNA sequence 5'-d(CAC)₄/5'-d(GTG)₄ are compared with DNA single strand breaks in the oligonucleotides 5'-d(CAC)₄ and 5'-d(GTG)₄ upon irradiation with LEEs in the energy range from 5 to 20 eV. A maximum of strand break cross section wa

SUBMITTER: Ebel K 

PROVIDER: S-EPMC9189919 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature