Intestinal Epithelial Expression of MHCII Determines Severity of Chemical, T-Cell-Induced, and Infectious Colitis in Mice.

ABSTRACT:

Background & aims

Intestinal epithelial cells (IECs) provide a barrier that separates the mucosal immune system from the luminal microbiota. IECs constitutively express low levels of major histocompatibility complex (MHC) class II proteins, which are upregulated upon exposure to interferon gamma. We investigated the effects of deleting MHCII proteins specifically in mice with infectious, dextran sodium sulfate (DSS)-, and T-cell-induced colitis.

Methods

We disrupted the histocompatibility 2, class II antigen A, beta 1 gene (H2-Ab1) in IECs of C57BL/6 mice (I-Ab^ΔIEC) or Rag1^-/- mice (Rag1^-/-I-Ab^ΔIEC); we used I-Ab^WT mice as controls. Colitis was induced by administration of DSS, transfer of CD4⁺CD45RB^hi

SUBMITTER: Jamwal DR 

PROVIDER: S-EPMC9190026 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature