Dynamics of metal binding and mutation in yybP-ykoY riboswitch of Lactococcus lactis.

ABSTRACT: Riboswitch is a regulatory segment of messenger RNA (mRNA), which by binding to various cellular metabolites regulates the activity of mRNA via modulating transcription, translation, alternative splicing, and stability of the mRNA. yybP-ykoY riboswitch of Lactococcus lactis, which is present upstream of the yoaB gene, functions as a Mn²⁺-specific genetic ON-switch, and modulates expression of proteins which are significant for Mn²⁺ homeostasis. The P1.1 switch helix of the aptamer domain of the riboswitch contains an intrinsic transcription terminator structure, which gets stabilized with Mn²⁺ binding and causes disruption of terminator structure and allows the continuation of transcription. The current research work involved the evaluation of

SUBMITTER: Iqbal M 

PROVIDER: S-EPMC9190785 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature