Layer-specific nanophotonic delivery of therapeutic opsin-encoding genes into retina.

ABSTRACT: In recent time, gene therapy has proven to be a promising remedial approach for treating visual disorders either by replacement of nonfunctioning gene(s) or by introduction of light sensitive proteins (opsins) as artificial photoreceptors in retinal cells. Conventional viral vector-based gene delivery method is often confronted with limitations due to immunogenetic reaction, unintended non-targeted delivery, non-feasibility of repeated re-dosing due to immunorejection, and complicated manufacturing process, leading to significant roadblock in translational success. In this regard, non-viral delivery provides a safer, simpler and cost-effective alternative. However, most of the non-viral approaches lack spatial and/or cellular specificity and limited by low transfection efficacy and cytotox

SUBMITTER: Batabyal S 

PROVIDER: S-EPMC9191255 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature