Elevated Temperatures Cause Transposon-Associated DNA Damage in C. elegans Spermatocytes.

ABSTRACT: Sexually reproducing organisms use meiosis to generate haploid gametes and faithfully transmit their genome to the next generation. In comparison to oogenesis in many organisms, spermatogenesis is particularly sensitive to small temperature fluctuations, and spermatocytes must develop within a very narrow isotherm [1-4]. Although failure to thermoregulate spermatogenetic tissue and prolonged exposure to elevated temperatures are linked to male infertility in several organisms, the mechanisms of temperature-induced male infertility have not been fully elucidated [5]. Here, we show that upon exposure to a brief 2°C temperature increase, Caenorhabditis elegans spermatocytes exhibit up to a 25-fold increase in double-strand DNA breaks (DSBs) throughout meiotic prophase I and a concurrent reduc

SUBMITTER: Kurhanewicz NA 

PROVIDER: S-EPMC9191688 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature