DNP-enhanced solid-state NMR spectroscopy of chromatin polymers.

ABSTRACT: Chromatin is a DNA-protein polymer that represents the functional form of the genome. The main building block of chromatin is the nucleosome, a structure that contains 147 base pairs of DNA and two copies each of the histone proteins H2A, H2B, H3 and H4. Previous work has shown that magic angle spinning (MAS) NMR spectroscopy can capture the nucleosome at high resolution although studies have been challenging due to low sensitivity, the presence of dynamic and rigid components, and the complex interaction networks of nucleosomes within the chromatin polymer. Here, we use dynamic nuclear polarization (DNP) to enhance the sensitivity of MAS NMR experiments of nucleosome arrays at 100 K and show that well-resolved ¹³C-¹³C MAS NMR correlations can be obtained much more ef

SUBMITTER: Elathram N 

PROVIDER: S-EPMC9191766 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature