Sensitive and accurate analysis of gene expression signatures enabled by oligonucleotide-labelled cDNA.

ABSTRACT: High-throughput RNA sequencing offers a comprehensive analysis of transcriptome complexity originated from regulatory events, such as differential gene expression, alternative polyadenylation and others, and allows the increase in diagnostic capacity and precision. For gene expression profiling applications that do not specifically require information on alternative splicing events, the mRNA 3' termini counting approach is a cost-effective alternative to whole transcriptome sequencing. Here, we report MTAS-seq (mRNA sequencing via terminator-assisted synthesis) - a novel RNA-seq library preparation method directed towards mRNA 3' termini. We demonstrate the specific enrichment for 3'-terminal regions by simple and quick single-tube protocol with built-in molecular barcoding to enable accur

SUBMITTER: Kapustina Z 

PROVIDER: S-EPMC9191874 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature