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Perception of a conserved family of plant signalling peptides by the receptor kinase HSL3.

ABSTRACT: Plant genomes encode hundreds of secreted peptides; however, relatively few have been characterised. We report here an uncharacterised, stress-induced family of plant signalling peptides, which we call CTNIPs. Based on the role of the common co-receptor BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED KINASE 1 (BAK1) in CTNIP-induced responses, we identified in Arabidopsis thaliana the orphan receptor kinase HAESA-LIKE 3 (HSL3) as the CTNIP receptor via a proteomics approach. CTNIP-binding, ligand-triggered complex formation with BAK1, and induced downstream responses all involve HSL3. Notably, the HSL3-CTNIP signalling module is evolutionarily conserved amongst most extant angiosperms. The identification of this novel signalling module will further shed light on the diverse functions played by plant signalling peptides and will provide insights into receptor-ligand co-evolution.

SUBMITTER: Rhodes J

PROVIDER: S-EPMC9191895 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Perception of a conserved family of plant signalling peptides by the receptor kinase HSL3.

Rhodes Jack J Roman Andra-Octavia AO Bjornson Marta M Brandt Benjamin B Derbyshire Paul P Wyler Michele M Schmid Marc W MW Menke Frank L H FLH Santiago Julia J Zipfel Cyril C

eLife 20220526

Plant genomes encode hundreds of secreted peptides; however, relatively few have been characterised. We report here an uncharacterised, stress-induced family of plant signalling peptides, which we call CTNIPs. Based on the role of the common co-receptor BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED KINASE 1 (BAK1) in CTNIP-induced responses, we identified in <i>Arabidopsis thaliana</i> the orphan receptor kinase HAESA-LIKE 3 (HSL3) as the CTNIP receptor via a proteomics approach. CTNIP-binding, ligan ...[more]

PMID: 35617122

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Perception of a conserved family of plant signaling peptides by the receptor kinase HSL3

Project description:Plant genomes encode hundreds of secreted peptides; however, relatively few have been characterised. We report here an uncharacterised, stress-induced family of plant signalling peptides, which we call CTNIPs. Based on the role of the common co-receptor BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED KINASE 1 (BAK1) in CTNIP-induced responses, we identified the orphan receptor kinase HAESA-LIKE 3 (HSL3) as the CTNIP receptor via a proteomics approach. CTNIP binding, ligand-triggered complex formation with BAK1, and induced downstream responses all involve HSL3. Notably, the HSL3-CTNIP signalling module is evolutionary ancient, predating the divergence of extant angiosperms. Definition of this signalling module will help establish its physiological role and provides a resource to understand further receptor-ligand co-evolution.

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