Phosphoproteomic identification of vasopressin-regulated protein kinases in collecting duct cells.

ABSTRACT:

Background and purpose

The peptide hormone vasopressin regulates water transport in the renal collecting duct largely via the V₂ receptor, which triggers a cAMP-mediated activation of a PKA-dependent signalling network. The protein kinases downstream from PKA have not been fully identified or mapped to regulated phosphoproteins.

Experimental approach

We carried out systems-level analysis of large-scale phosphoproteomic data quantifying vasopressin-induced changes in phosphorylation in aquaporin-2-expressing cultured collecting duct (mpkCCD) cells. Quantification was done using stable isotope labelling (SILAC method).

Key results

Six hundred forty phosphopeptides were quantified. Stringent statistical analysis identified significant changes in response to va

SUBMITTER: Datta A 

PROVIDER: S-EPMC9192144 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature