Luseogliflozin preserves the pancreatic beta-cell mass and function in db/db mice by improving mitochondrial function.

ABSTRACT: We aimed to determine the mechanism by which the sodium glucose co-transporter 2 inhibitor, luseogliflozin, preserves pancreatic beta-cell mass and function in db/db mice. Six-week-old db/db mice were fed to standard chow or standard chow containing 0.01% luseogliflozin. After 4 weeks, DNA microarray analysis, real-time PCR analysis, and measurement of mitochondrial respiratory capacity and reactive oxygen species (ROS) generation were performed using isolated islets. Immunohistochemistry and electron microscopic analysis were performed using pancreatic tissues. Metabolites extracted from the islets were measured by capillary electrophoresis mass spectrometry. The expression of genes involved in the tricarboxylic acid (TCA) cycle and electron transport chain was upregulated by luseoglifloz

SUBMITTER: Yamauchi Y 

PROVIDER: S-EPMC9192642 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature