Humanized yeast to model human biology, disease and evolution.

ABSTRACT: For decades, budding yeast, a single-cellular eukaryote, has provided remarkable insights into human biology. Yeast and humans share several thousand genes despite morphological and cellular differences and over a billion years of separate evolution. These genes encode critical cellular processes, the failure of which in humans results in disease. Although recent developments in genome engineering of mammalian cells permit genetic assays in human cell lines, there is still a need to develop biological reagents to study human disease variants in a high-throughput manner. Many protein-coding human genes can successfully substitute for their yeast equivalents and sustain yeast growth, thus opening up doors for developing direct assays of human gene function in a tractable system referred to a

SUBMITTER: Kachroo AH 

PROVIDER: S-EPMC9194483 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature