Sphingosine kinase 1 mediates sexual dimorphism in fibrosis in a mouse model of NASH.

ABSTRACT:

Objective

Men with non-alcoholic fatty liver disease (NAFLD) are more likely to progress to non-alcoholic steatohepatitis (NASH) and liver fibrosis than women. However, the underlying molecular mechanisms of this dimorphism is unclear. We have previously shown that mice with global deletion of SphK1, the enzyme that produces the bioactive sphingolipid metabolite sphingosine 1-phosphate (S1P), were protected from development of NASH. The aim of this study was to elucidate the role of hepatocyte-specific SphK1 in development of NASH and to compare its contribution to hepatosteatosis in male and female mice.

Methods

We assessed mouse livers in early-stage fibrosis induced by high fat feeding, using single harmonic generation microscopy, LC-MS/MS analysis of hydroxyproline level

SUBMITTER: Montefusco D 

PROVIDER: S-EPMC9194589 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature