Coexistence of bla _NDM-1 and bla _IMP-4 in One Novel Hybrid Plasmid Confers Transferable Carbapenem Resistance in an ST20-K28 Klebsiella pneumoniae.

ABSTRACT:

Objectives

We identified a novel hybrid plasmid simultaneously carrying bla _NDM-1 and bla _IMP-4 in an ST20-K28 carbapenem-resistant Klebsiella pneumoniae (CRKP) strain AZS099 and reported its detailed genetic and phenotypic characterization.

Methods

Antimicrobial susceptibility was characterized using broth microdilution method. Complete genome characteristics and plasmid detailed analysis were carried out by PacBio Sequel and Illumina sequencing and further bioinformatics analysis. Conjugation assay, S1-PFGE, Southern blot, plasmid stability, and fitness cost were conducted to the phenotypic characterization of this novel hybrid plasmid.

Results

AZS099 was isolated from a blood specimen obtained from a 3-month baby who prese

SUBMITTER: Jia X 

PROVIDER: S-EPMC9194606 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature