Novel switchable ECF sigma factor transcription system for improving thaxtomin A production in Streptomyces.

ABSTRACT: The application of the valuable natural product thaxtomin A, a potent bioherbicide from the potato scab pathogenic Streptomyces strains, has been greatly hindered by the low yields from its native producers. Here, we developed an orthogonal transcription system, leveraging extra-cytoplasmic function (ECF) sigma (σ) factor 17 (ECF17) and its cognate promoter P_ecf17, to express the thaxtomin gene cluster and improve the production of thaxtomin A. The minimal P_ecf17 promoter was determined, and a P_ecf17 promoter library with a wide range of strengths was constructed. Furthermore, a cumate inducible system was developed for precise temporal control of the ECF17 transcription system in S. venezuelae ISP5230. Theoretically, the switchable ECF17 tran

SUBMITTER: Zhao X 

PROVIDER: S-EPMC9194655 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature