Leptin favors Th17/Treg cell subsets imbalance associated with allergic asthma severity.

ABSTRACT:

Background

Obesity has often been associated with severe allergic asthma (AA). Here, we analyzed the frequency of different circulating CD4⁺T-cell subsets from lean, overweight and obese AA patients.

Methods

Mononuclear cells from peripheral blood were obtained from 60 AA patients and the frequency of different CD4⁺T-cell subsets and type 1 regulatory B cells (Br1) was determined by cytometry. The effect of obese-related leptin dose on cytokine production and Treg cell function in AA-derived CD4⁺ T cell cultures was evaluated by ELISA and 3H thymidine uptake, respectively. Leptin levels were quantified in the plasma by ELISA. According to the BMI, patients were stratified as lean, overweight and obese.

Results

AA severity, mainly among

SUBMITTER: Vollmer CM 

PROVIDER: S-EPMC9194742 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature