Fe³⁺-NTA magnetic beads as an alternative to spin column-based phosphopeptide enrichment.

ABSTRACT: Protein phosphorylation is a central mechanism of cellular signal transduction in living organisms. Phosphoproteomic studies systematically catalogue and characterize alterations in phosphorylation states across multiple cellular conditions and are often incorporated into global proteomics experiments. Previously, we found that spin column-based Fe³⁺-NTA enrichment integrated well with our workflow but remained a bottleneck for methods that require higher throughput or a scale that is beyond the capacity of these columns. Here, we compare our well-established spin column-based enrichment strategy with one encompassing magnetic beads. Our data show little difference when using either method in terms of the number of identified phosphopeptides as well as their physicochemical prop

SUBMITTER: Liu X 

PROVIDER: S-EPMC9195054 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature