Adenovirus-Vectored African Swine Fever Virus pp220 Induces Robust Antibody, IFN-γ, and CTL Responses in Pigs.

ABSTRACT: African Swine Fever Virus (ASFV) poses a serious threat to the pork industry worldwide; however, there is no safe vaccine or treatment available. The development of an efficacious subunit vaccine will require the identification of protective antigens. The ASFV pp220 polyprotein is essential for virus structural integrity. This polyprotein is processed to generate p5, p34, p14, p37, and p150 individual proteins. Immunization of pigs with a cocktail of adenoviruses expressing the proteins induced significant IgG, IFN-γ-secreting cells, and cytotoxic T lymphocyte responses. Four predicted SLA-I binding nonamer peptides, namely p34^161-169, p37^859-867, p150^1363-1371, and p150^1463-1471, recalled strong IFN-γ⁺ PBMC and splenocyte respo

SUBMITTER: Zajac MD 

PROVIDER: S-EPMC9195138 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature