Breadth of Antibodies to <i>Plasmodium falciparum</i> Variant Surface Antigens Is Associated With Immunity in a Controlled Human Malaria Infection Study.

ABSTRACT:

Background

Plasmodium falciparum variant surface antigens (VSAs) contribute to malaria pathogenesis by mediating cytoadhesion of infected red blood cells to the microvasculature endothelium. In this study, we investigated the association between anti-VSA antibodies and clinical outcome in a controlled human malaria infection (CHMI) study.

Method

We used flow cytometry and ELISA to measure levels of IgG antibodies to VSAs of five heterologous and one homologous P. falciparum parasite isolates, and to two PfEMP1 DBLβ domains in blood samples collected a day before the challenge and 14 days after infection. We also measured the ability of an individual's plasma to inhibit the interaction between PfEMP1 and ICAM1 using competition ELISA. We then assessed the associ

SUBMITTER: Kimingi HW 

PROVIDER: S-EPMC9195513 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature