Systematic characterization of effect of flow rates and buffer compositions on double emulsion droplet volumes and stability.

ABSTRACT: Double emulsion droplets (DEs) are water/oil/water droplets that can be sorted via fluorescence-activated cell sorting (FACS), allowing for new opportunities in high-throughput cellular analysis, enzymatic screening, and synthetic biology. These applications require stable, uniform droplets with predictable microreactor volumes. However, predicting DE droplet size, shell thickness, and stability as a function of flow rate has remained challenging for monodisperse single core droplets and those containing biologically-relevant buffers, which influence bulk and interfacial properties. As a result, developing novel DE-based bioassays has typically required extensive initial optimization of flow rates to find conditions that produce stable droplets of the desired size and shell thicknes

SUBMITTER: Calhoun SGK 

PROVIDER: S-EPMC9195911 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature