Extremophile Metal Resistance: Plasmid-Encoded Functions in Streptomyces mirabilis.

ABSTRACT: The extreme metal tolerance of up to 130 mM NiSO₄ in Streptomyces mirabilis P16B-1 was investigated. Genome sequencing revealed the presence of a large linear plasmid, pI. To identify plasmid-encoded determinants of metal resistance, a newly established transformation system was used to characterize the predicted plasmid-encoded loci nreB, hoxN, and copYZ. Reintroduction into the plasmid-cured S. mirabilis ΔpI confirmed that the predicted metal transporter gene nreB constitutes a nickel resistance factor, which was further supported by its heterologous expression in Escherichia coli. In contrast, the predicted nickel exporter gene hoxN decreased nickel tolerance, while copper tolerance was enhanced. The predicted copper-dependent transcriptional regul

SUBMITTER: Brangsch H 

PROVIDER: S-EPMC9195940 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature