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Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies.

ABSTRACT: Here, we demonstrate that by combining electroporation with native ambient mass spectrometry, it is possible to detect intact non-covalent protein complexes directly from bacterial colonies growing on agar. Homodimers HdeA and HdeB were identified, together with the 50 kDa Mn-bound superoxide dismutase homodimer, in addition to some previously undetected monomeric proteins.

SUBMITTER: Du Y

PROVIDER: S-EPMC9196203 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature

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Native ambient mass spectrometry of intact protein assemblies directly from <i>Escherichia coli</i> colonies.

Du Yuying Y May Robin C RC Cooper Helen J HJ

Chemical communications (Cambridge, England) 20220614 48

Here, we demonstrate that by combining electroporation with native ambient mass spectrometry, it is possible to detect intact non-covalent protein complexes directly from bacterial colonies growing on agar. Homodimers HdeA and HdeB were identified, together with the 50 kDa Mn-bound superoxide dismutase homodimer, in addition to some previously undetected monomeric proteins. ...[more]

PMID: 35617055

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