Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies.

ABSTRACT: Here, we demonstrate that by combining electroporation with native ambient mass spectrometry, it is possible to detect intact non-covalent protein complexes directly from bacterial colonies growing on agar. Homodimers HdeA and HdeB were identified, together with the 50 kDa Mn-bound superoxide dismutase homodimer, in addition to some previously undetected monomeric proteins.

SUBMITTER: Du Y 

PROVIDER: S-EPMC9196203 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature