NtEdit+Sealer: Efficient Targeted Error Resolution and Automated Finishing of Long-Read Genome Assemblies.

ABSTRACT: High-quality genome assemblies are crucial to many biological studies, and utilizing long sequencing reads can help achieve higher assembly contiguity. While long reads can resolve complex and repetitive regions of a genome, their relatively high associated error rates are still a major limitation. Long reads generally produce draft genome assemblies with lower base quality, which must be corrected with a genome polishing step. Hybrid genome polishing solutions can greatly improve the quality of long-read genome assemblies by utilizing more accurate short reads to validate bases and correct errors. Currently available hybrid polishing methods rely on read alignments, and are therefore memory-intensive and do not scale well to large genomes. Here we describe ntEdit+Sealer, an alignment-free

SUBMITTER: Li JX 

PROVIDER: S-EPMC9196995 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature