Learning the statistics and landscape of somatic mutation-induced insertions and deletions in antibodies.

ABSTRACT: Affinity maturation is crucial for improving the binding affinity of antibodies to antigens. This process is mainly driven by point substitutions caused by somatic hypermutations of the immunoglobulin gene. It also includes deletions and insertions of genomic material known as indels. While the landscape of point substitutions has been extensively studied, a detailed statistical description of indels is still lacking. Here we present a probabilistic inference tool to learn the statistics of indels from repertoire sequencing data, which overcomes the pitfalls and biases of standard annotation methods. The model includes antibody-specific maturation ages to account for variable mutational loads in the repertoire. After validation on synthetic data, we applied our tool to a large dataset of h

SUBMITTER: Lupo C 

PROVIDER: S-EPMC9197026 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature