Unveiling the Role of DNA Methylation in Vascular CACNA1C Tissue-Specific Expression.

ABSTRACT:

Objective

Calcium voltage-gated channel subunit alpha1 C (CACNA1C) plays a critical role in many vascular physiological and pathological processes. Determining its tissue-specific expression pattern and clarifying the underlying molecular mechanisms are necessary and meaningful.

Methods

We selected several representative vessels from normal male Sprague-Dawley rats. Vessel tissue or primary vascular smooth muscle cells were isolated for vascular function, electrophysiology, gene expression and promoter methylation studies.

Results

We found CACNA1C had tissue-specific expressions in vessels. The specific manifestations were as follows: CACNA1C expression was highest in thoracic aorta, second lowest in middle cerebral and pulmonary artery, and lowest in mesenteric arter

SUBMITTER: Zhao M 

PROVIDER: S-EPMC9197502 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature