Effects of Platelet Agonists and Priming on the Formation of Platelet Populations.

ABSTRACT: Platelets from healthy donors display heterogeneity in responsiveness to agonists. The response thresholds of platelets are controlled by multiple bioactive molecules, acting as negatively or positively priming substances. Higher circulating levels of priming substances adenosine and succinate, as well as the occurrence of hypercoagulability, have been described for patients with ischaemic heart disease. Here, we present an improved methodology of flow cytometric analyses of platelet activation and the characterisation of platelet populations following activation and priming by automated clustering analysis.Platelets were treated with adenosine, succinate, or coagulated plasma before stimulation with CRP-XL, 2-MeSADP, or TRAP6 and labelled for activated integrin α_IIbβ₃

SUBMITTER: Veninga A 

PROVIDER: S-EPMC9197595 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature