Spinach-based RNA mimicking GFP in plant cells.

ABSTRACT: Spinach RNA-mimicking GFP (S-RMG) has been successfully used to monitor cellular RNAs including microRNAs in bacterium, yeast, and human cells. However, S-RMG has not been established in plants. In this study, we found that like bacterial, yeast, and human cellular tRNAs, plant tRNAs such as tRNA^Lys can protect and/or stabilize the Spinach RNA aptamer interaction with the fluorophore DFHBI enabling detectable levels of green fluorescence to be emitted. The tRNA^Lys-Spinach-tRNA^Lys, once delivered into "chloroplast-free" onion epidermal cells can emit strong green fluorescence in the presence of DFHBI. Our results demonstrate for the first time that Spinach-based RNA visualization has the potential for in vivo monitoring of RNAs in plant cells.

SUBMITTER: Yu Z 

PROVIDER: S-EPMC9197860 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature