Nox4 expression in osteo-progenitors controls bone development in mice during early life.

ABSTRACT: Tightly regulated and cell-specific NADPH-oxidases (Nox) represent one of the major sources of reactive oxygen species (ROS) signaling molecules that are involved in tissue development and stem cell self-renewal. We have characterized the role of Nox4 in osteo-progenitors during postnatal bone development. Nox4 expression in bone and ROS generation were increased during early osteoblast differentiation and bone development. Stromal osteoblastic cell self-renewal, proliferation and ROS production were significantly lower in samples from whole-body Nox4 knockout mice (Nox4^-/-) and conditional knockout (CKO) mice with depletion of Nox4 in the limb bud mesenchyme compared with those from control mice (Nox4^fl/fl), but they were reversed after 9 passages. In both sexes, bon

SUBMITTER: Chen JR 

PROVIDER: S-EPMC9198054 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature