Establishment and characterization of a novel human induced pluripotent stem cell line stably expressing the iRFP720 reporter.

ABSTRACT: Stem cell therapy has great potential for replacing beta-cell loss in diabetic patients. However, a key obstacle to cell therapy's success is to preserve viability and function of the engrafted cells. While several strategies have been developed to improve engrafted beta-cell survival, tools to evaluate the efficacy within the body by imaging are limited. Traditional labeling tools, such as GFP-like fluorescent proteins, have limited penetration depths in vivo due to tissue scattering and absorption. To circumvent this limitation, a near-infrared fluorescent mutant version of the DrBphP bacteriophytochrome, iRFP720, has been developed for in vivo imaging and stem/progenitor cell tracking. Here, we present the generation and characterization of an iRFP720 expressing human induced pluripoten

SUBMITTER: Feher A 

PROVIDER: S-EPMC9198085 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature