Functional characterization of piggyBac-like elements from Nilaparvata lugens (Stal) (Hemiptera: Delphacidae).

ABSTRACT: PiggyBac is a transposable DNA element originally discovered in the cabbage looper moth (Trichoplusia ni). The T. ni piggyBac transposon can introduce exogenous fragments into a genome, constructing a transgenic organism. Nevertheless, the comprehensive analysis of endogenous piggyBac-like elements (PLEs) is important before using piggyBac, because they may influence the genetic stability of transgenic lines. Herein, we conducted a genome-wide analysis of PLEs in the brown planthopper (BPH) Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), and identified a total of 28 PLE sequences. All N. lugens piggyBac-like elements (NlPLEs) were present as multiple copies in the genome of BPH. Among the identified

SUBMITTER: Lyu J 

PROVIDER: S-EPMC9198232 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature