Cardiac fibroblast sub-types in vitro reflect pathological cardiac remodeling in vivo.

ABSTRACT: Many heart diseases are associated with fibrosis, but it is unclear whether different types of heart disease correlate with different subtypes of activated fibroblasts and to which extent such diversity is modeled during in vitro activation of primary cardiac fibroblasts. Analyzing the expression of 82 fibrosis related genes in 65 heart failure (HF) patients, we identified a panel of 12 genes clearly distinguishing HF patients better from healthy controls than measurement of the collagen-related hydroxyproline content. A subcluster enriched in ischemic HF was recognized, but not for diabetes, high BMI, or gender. Single-cell transcriptomic analysis of in vitro activated mouse cardiac fibroblasts distinguished 6 subpopulations, including a contractile Acta2^high prec

SUBMITTER: Herum KM 

PROVIDER: S-EPMC9198323 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature