Crosstalk between IL-15Rα⁺ tumor-associated macrophages and breast cancer cells reduces CD8⁺ T cell recruitment.

ABSTRACT:

Background

Interleukin-15 (IL-15) is a promising immunotherapeutic agent owing to its powerful immune-activating effects. However, the clinical benefits of these treatments are limited. Crosstalk between tumor cells and immune cells plays an important role in immune escape and immunotherapy drug resistance. Herein, this study aimed to obtain in-depth understanding of crosstalk in the tumor microenvironment for providing potential therapeutic strategies to prevent tumor progression.

Methods

T-cell killing assays and co-culture models were developed to determine the role of crosstalk between macrophages and tumor cells in breast cancer resistant to IL-15. Western blotting, histological analysis, CRISPR-Cas9 knockout, multi-parameter flow cytometry, and tumor cell-macrophage co

SUBMITTER: Zhang W 

PROVIDER: S-EPMC9198341 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature