Transplantation of human cells into Interleukin-2 receptor gamma gene knockout pigs under several conditions.

ABSTRACT:

Introduction

Previously, we performed gene knockout (KO) of interleukin-2 receptor gamma (IL2RG) in porcine fetal fibroblasts using zinc finger nuclease-encoding mRNAs, subsequently generating IL2RG KO pigs using these cells through somatic cell nuclear transfer. The IL2RG KO pigs lacked a thymus and were deficient in T lymphocytes and natural killer cells, similar to human X-linked severe combined immunodeficiency (SCID) patients. The present study aimed to evaluate whether pigs can support the growth of xenografted human cells and have the potential to be an effective animal model.

Methods

The IL2RG X^KOY pigs used in this study were obtained by mating IL2RG X^KOX females with wild-type boars. This permitted the routin

SUBMITTER: Hasegawa K 

PROVIDER: S-EPMC9198816 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature