3D Lung-on-Chip Model Based on Biomimetically Microcurved Culture Membranes.

ABSTRACT: A comparatively straightforward approach to accomplish more physiological realism in organ-on-a-chip (OoC) models is through substrate geometry. There is increasing evidence that the strongly, microscale curved surfaces that epithelial or endothelial cells experience when lining small body lumens, such as the alveoli or blood vessels, impact their behavior. However, the most commonly used cell culture substrates for modeling of these human tissue barriers in OoCs, ion track-etched porous membranes, provide only flat surfaces. Here, we propose a more realistic culture environment for alveolar cells based on biomimetically microcurved track-etched membranes. They recreate the mainly spherical geometry of the cells' native microenvironment. In this feasibility study, the membranes were given

SUBMITTER: Baptista D 

PROVIDER: S-EPMC9198974 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature