Measurement of the persistence length of cytoskeletal filaments using curvature distributions.

ABSTRACT: Cytoskeletal filaments, such as microtubules and actin filaments, play important roles in the mechanical integrity of cells and the ability of cells to respond to their environment. Measuring the mechanical properties of cytoskeletal structures is crucial for gaining insight into intracellular mechanical stresses and their role in regulating cellular processes. One of the ways to characterize these mechanical properties is by measuring their persistence length, the average length over which filaments stay straight. There are several approaches in the literature for measuring filament deformations, such as Fourier analysis of images obtained using fluorescence microscopy. Here, we show how curvature distributions can be used as an alternative tool to quantify biofilament deformations, and i

SUBMITTER: Wisanpitayakorn P 

PROVIDER: S-EPMC9199094 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature