Osmotic Processor for Enabling Sensitive and Rapid Biomarker Detection via Lateral Flow Assays.

ABSTRACT: Urine is an attractive biospecimen for in vitro diagnostics, and urine-based lateral flow assays are low-cost devices suitable for point-of-care testing, particularly in low-resource settings. However, some of the lateral flow assays exhibit limited diagnostic utility because the urinary biomarker concentration is significantly lower than the assay detection limit, which compromises the sensitivity. To address the challenge, we developed an osmotic processor that statically and spontaneously concentrated biomarkers. The specimen in the device interfaces with the aqueous polymer solution via a dialysis membrane. The polymer solution induces an osmotic pressure difference that extracts water from the specimen, while the membrane retains the biomarkers. The evaluation demonstrated that

SUBMITTER: Chen SY 

PROVIDER: S-EPMC9199386 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature