Advances and Challenges in Fluorescence in situ Hybridization for Visualizing Fungal Endobacteria.

ABSTRACT: Several bacteria have long been known to interact intimately with fungi, but molecular approaches have only recently uncovered how cosmopolitan these interactions are in nature. Currently, bacterial-fungal interactions (BFI) are inferred based on patterns of co-occurrence in amplicon sequencing investigations. However, determining the nature of these interactions, whether the bacteria are internally or externally associated, remains a grand challenge in BFI research. Fluorescence in situ hybridization (FISH) is a robust method that targets unique sequences of interest which can be employed for visualizing intra-hyphal targets, such as mitochondrial organelles or, as in this study, bacteria. We evaluate the challenges and employable strategies to resolve intra-hyphal BFI to address p

SUBMITTER: Morales DP 

PROVIDER: S-EPMC9199388 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature