Novel MicroRNAs and their Functional Targets from Phytophthora infestans and Phytophthora cinnamomi.

ABSTRACT:

Background

Even though miRNAs play vital roles in developmental biology by regulating the translation of mRNAs, they are poorly studied in oomycetes, especially in the plant pathogen Phytophthora.

Objective

The study aimed to predict and identify the putative miRNAs and their targets in Phytophthora infestans and Phytophthora cinnamomi.

Methods

The homology-based comparative method was used to identify the unique miRNA sequences in P. infestans and P. cinnamomi with 148,689 EST and TSA sequences of these species. Secondary structure prediction of sRNAs for the 76 resultant sequences has been performed with the MFOLD tool, and their targets were predicted using psRNATarget.

Results

Novel miRNAs, miR-8210 and miR-4968, were pred

SUBMITTER: Varghese B 

PROVIDER: S-EPMC9199537 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature