Parallel imaging of coagulation pathway proteases activated protein C, thrombin, and factor Xa in human plasma.

ABSTRACT: Activated protein C (APC), thrombin, and factor (f) Xa are vitamin K-dependent serine proteases that are key factors in blood coagulation. Moreover, they play important roles in inflammation, apoptosis, fibrosis, angiogenesis, and viral infections. Abnormal activity of these coagulation factors has been related to multiple conditions, such as bleeding and thrombosis, Alzheimer's disease, sepsis, multiple sclerosis, and COVID-19. The individual activities of APC, thrombin, and fXa in coagulation and in various diseases are difficult to establish since these proteases are related and have similar substrate preferences. Therefore, the development of selective chemical tools that enable imaging and discrimination between coagulation factors in biological samples may provide better insight into

SUBMITTER: Modrzycka S 

PROVIDER: S-EPMC9200056 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature