Evaluation of Whatman FTA cards for the preservation of yellow fever virus RNA for use in molecular diagnostics.

ABSTRACT: Yellow fever virus (YFV) is a flavivirus that frequently causes outbreaks of hemorrhagic fever in Africa and South America and is considered a reemerging public health threat. Accurate diagnosis of yellow fever (YF) disease is critical as one confirmed case constitutes an outbreak and may trigger a mass vaccination campaign. Highly sensitive and specific molecular diagnostics have been developed; however, these assays require maintenance of cold-chain during transport of specimens to prevent the degradation of viral RNA prior to testing. Such cold-chain requirements are difficult to meet in some regions. In this study, we investigated Whatman FTA cards as an alternative stabilization method of YFV RNA for use in molecular diagnosis. Using contrived specimens, linear regression analysis sho

SUBMITTER: Davis EH 

PROVIDER: S-EPMC9200311 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature