Differential Regulation of the Immune System in Peripheral Blood Following Ischemic Stroke.

ABSTRACT:

Method

108 IS samples and 47 matched controls were obtained from the GEO database. Immune-related genes (IRGs) and their associated drugs were collected from the ImmPort and PharmGBK databases, respectively. Random forest (RF) regression and least absolute shrinkage and selection operator (LASSO) logistic regression were applied to identify immune-related genetic biomarkers (IRGBs) of IS, and accuracy was verified using neural network models. Finally, proportion changes of various immune cells in peripheral blood of IS patients were evaluated using CIBERSORT and xCell and correlation analyses were performed between IRGBs and differentially distributed immune cells.

Results

A total of 537 genes were differentially expressed between IS and control samples. Four immune-related

SUBMITTER: Liu W 

PROVIDER: S-EPMC9200570 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature