Ly6D⁺Siglec-H⁺ precursors contribute to conventional dendritic cells via a Zbtb46⁺Ly6D⁺ intermediary stage.

ABSTRACT: Plasmacytoid and conventional dendritic cells (pDC and cDC) are generated from progenitor cells in the bone marrow and commitment to pDCs or cDC subtypes may occur in earlier and later progenitor stages. Cells within the CD11c⁺MHCII^-/loSiglec-H⁺CCR9^lo DC precursor fraction of the mouse bone marrow generate both pDCs and cDCs. Here we investigate the heterogeneity and commitment of subsets in this compartment by single-cell transcriptomics and high-dimensional flow cytometry combined with cell fate analysis: Within the CD11c⁺MHCII^-/loSiglec-H⁺CCR9^lo DC precursor pool cells expressing high levels of Ly6D and lacking expression of transcription factor Zbtb46 contain CCR9^loB220^hi immed

SUBMITTER: Lutz K 

PROVIDER: S-EPMC9200809 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature